The Cross-validation of the 122 catagorized kinase families The experimentally verified phosphorylated sites are collected from PhosphoELM and SwissProt. All the 518 kinase genes which were identified by Manning et al. are categorized by their annotated family or subfamily. There are totally 122 kinase familes including more than 300 kinases. The number of phosphorylated sites in human proteins is listed, as well as the number of phosphorylated proteins. Moreover, the sequence logo of each kinase family is presented by using Weblogo. Prediction by KinasePhos 2.0

No.	Group	Family	Subfamily	Family description	Kinase member	Number of phosphosites in all species	Sequence logo	HMM Sn.	HMM Sp.	HMM Acc.
1	AGC	Akt		v-akt murine thymoma viral oncogene homolog	AKT1 AKT2 AKT3	197		98.1	94.1	96.1
2	AGC	DMPK	ROCK	Rho Kinase	ROCK1 ROCK2	97		90.9	89.1	90
3	AGC	GEK	GEK	nan	DMPK MRCKa	19		94.7	97.4	96
4	AGC	GRK	GRK	G protein coupled Receptor Kinase	GPRK7 RHOK GPRK6 GPRK5 GPRK4	82		81.6	79.5	80.6
5	AGC	GRK	BARK	Beta Adrenergic Receptor Kinase	BARK1 BARK2	39		80.7	83.1	81.9
6	AGC	NDR		Nuclear Dbf2related kinases	NDR1 NDR2 LATS1 LATS2	16		93.8	93.8	93.8
7	AGC	PKA		Protein kinase A	PKACa PKACb PKACg	546		89.2	89.4	89.3
8	AGC	PDK1		Phosphoinositidedependent protein kinase	PDK1	77		83.9	85.1	84.5
9	AGC	PKC		Protein kinase C	PKCh PKCa PKCb PKCd PKCe PKCg PKCi PKCt PKCz	652		85.9	87.2	86.6
10	AGC	PKC	Alpha	protein kinase C, alpha	PKCa PKCb PKCd PKCg	207		87.2	84.8	86
11	AGC	PKC	Delta	protein kinase C, delta	PKCd PKCt	42		89.9	90.2	90.1
12	AGC	PKC	Eta	protein kinase C, eta	PKCe PKCh	30		82.9	92	87.5
13	AGC	PKC	Iota	protein kinase C, iota	PKCi PKCz	28		85.9	87.2	86.6
14	AGC	PKG		Protein kinase G	PKG1 PKG2	64		89.2	91.1	90.1
15	AGC	PKN		Protein kinase N	PKN1 PKN2 PKN3	33		81.2	79.4	80.3
16	AGC	RSK	RSK	Ribosomal S6 Kinase	RSK1 RSK2 RSK3 RSK4	106		90.5	93.1	91.8
17	AGC	RSK	p70	p70 subfamily of Ribosomal Specific Kinase	p70S6K p70S6Kb	25		92	95.6	93.8
18	AGC	RSK	MSK	ribosomal protein S6 kinase, 90kDa, polypeptide	MSK1 MSK2	68		91.2	91.2	91.2
19	AGC	SGK		Serum and Glucocorticoid Resposive Kinase	SGK SGK2 SGK3	69		89.2	89.4	89.3
20	Atypical	Chak		nan	ChaK1 ChaK2	8		86.3	76.3	81.3
21	Atypical	PDHK		Pyruvate Dehydrogenase Kinase	PDHK1 PDHK2 PDHK3 PDHK4 BCKDK	21		90.3	92.4	91.4
22	Atypical	PIKK	ATM	Ataxia telangiectasia mutated	ATM ATR	181		99.1	96	97.6
23	Atypical	PIKK	DNAPK	DNA Protein Kinase	DNAPK	35		98.3	98.9	98.6
24	Atypical	PIKK	FRAP	FKBP12rapamycinassociated protein	FRAP	31		86.8	94.5	90.7
25	Atypical	TAF1		TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa	TAF1 TAF1L	8		82.5	87.5	85
26	CAMK	CAMK1		CAMK family 1	CaMK1a CaMK1b CaMK1g CaMK1d CaMK4	51		83.9	85.7	84.8
27	CAMK	CAMK2		CAMK family 2	CaMK2a CaMK2b CaMK2g CaMK2d	148		93	91.1	92.1
28	CAMK	CAMKL	NuaK	Novel (Nua) Kinase family	NuaK1 NuaK2	9		86.7	86.7	86.7
29	CAMK	CAMKL	QIK	SNF1-like kinase	QIK SIK	24		89.2	89.2	89.2
30	CAMK	CAMKL	MELK	maternal embryonic leucine zipper kinase	MELK	21		90	84.8	87.4
31	CAMK	CAMKL	AMPK	AMPactivated protein kinase	AMPKa1 AMPKa2	88		90.7	93.3	92
32	CAMK	CAMKL	CHK1	Checkpoint Kinase 1	CHK1	23		93.4	94	93.7
33	CAMK	CAMKL	CHK2	Checkpoint Kinase 2	CHK2	35		90.2	91.1	90.6
34	CAMK	CAMKL	LKB	serine/threonine kinase 11	LKB1	35		94.3	94.3	94.3
35	CAMK	CAMKL	MARK	Microtubule Affinity Regulating Kinase	MARK1 MARK2 MARK3 MARK4	24		91.7	91.7	91.7
36	CAMK	CAMKL	BRSK	BRain Specific Kinase	BRSK1	10		90.1	89.9	90
37	CAMK	DAPK		Death Associated Protein Kinase	DAPK1 DAPK2 DAPK3 DRAK1 DRAK2	64		93.3	95.4	94.4
38	CAMK	MAPKAPK	MAPKAPK	MAP Kinase Associated Protein Kinase	MAPKAPK2 MAPKAPK3 MAPKAPK5	68		89.6	91.9	90.8
39	CAMK	MAPKAPK	MNK	MAPK iNtegrating or iNteracting Kinase	MNK1 MNK2	11		100	96.3	98.2
40	CAMK	MLCK		Myosin Light Chain Kinases	smMLCK TTN caMLCK skMLCK Sgk085	21		91.4	93.3	92.4
41	CAMK	PHK		Phosphorylase Kinase	PHKg1 PHKg2	28		91.1	90.4	90.7
42	CAMK	PIM		pim-1 oncogene	PIM1 PIM2 PIM3	19		93.2	93.2	93.2
43	CAMK	PKD		Protein Kinase D	PKD1 PKD2 PKD3	32		96.6	92.5	94.5
44	CK1	CK1		Cell Kinase 1	CK1a CK1d CK1e CK1g2 CK1g3 CK1a2 CK1g1	267		92.2	90.9	91.6
45	CK1	TTBK		Tau Tubulin Kinase	TTBK1 TTBK2	10		90	100	95
46	CK1	VRK		Vaccinia Related Kinase	VRK1 VRK2 VRK3	16		87.5	87.5	87.5
47	CMGC	CDK		Cyclin Dependent Kinase	CDK4 CDK5 CDK6 CDK8 CDK9 CDK10 CDK11	193		95.2	90.7	93
48	CMGC	CDK	CDK7	Cyclin Dependent Kinase subfamily 7	CDK7	21		88.4	86.7	87.6
49	CMGC	CDK	CDC2	Cell Division Control 2	CDC2 CDK2 CDK3	436		94.3	90.5	92.4
50	CMGC	CK2		Casein kinase II	CK2 CK2a CK2b	488		87.1	88.5	87.8
51	CMGC	CLK		CDC-like kinase	CLK1 CLK2 CLK3 CLK4	12		86.7	86.7	86.7
52	CMGC	DYRK	HIPK	Homeodomain Interacting Protein Kinases	HIPK1 HIPK2 HIPK3 HIPK4	33		92.7	88.7	90.7
53	CMGC	DYRK	Dyrk	Dualspecificity Tyrosine Regulated Kinase	DYRK1A DYRK1B DYRK2 DYRK3 DYRK4	37		88.4	90.7	89.6
54	CMGC	GSK		Gycogen Synthase 3 Kinase	GSK3A GSK3B	148		83.6	80.1	81.8
55	CMGC	MAPK		Mitogen Activated Protein Kinase	Erk1(MAPK3) Erk2(MAPK1) Erk3(MAPK6) Erk4(MAPK4) Erk5(MAPK7) Erk7(MAPK15) JNK1(MAPK8) JNK2(MAPK9) JNK3(MAPK10) NLK p38a(MAPK14) p38b(MAPK11) p38g(MAPK12) p38d(MAPK13)	663		91.9	91.2	91.6
56	CMGC	MAPK	JNK	JNK subfamily of MAPK	JNK1(MAPK8) JNK2(MAPK9) JNK3(MAPK10)	111		92	92	92
57	CMGC	MAPK	p38	p38 subfamily of MAPK	p38a(MAPK14) p38b(MAPK11) p38g(MAPK12) p38d(MAPK13)	106		90	94.7	92.4
58	CMGC	MAPK	ERK	Extracellular signalRegulated protein Kinase	Erk1(MAPK3) Erk2(MAPK1) Erk3(MAPK6) Erk4(MAPK4) Erk5(MAPK7) Erk7(MAPK15)	357		92.8	91.1	92
59	CMGC	MAPK	nmo	nemo-like kinase	NLK	16		100	95.6	97.8
60	Other	AUR		Aurora Kinase	AurA AurB AurC	118		92.3	91.5	91.9
61	Other	AUR	AurB	Aurora Kinase B	AurB	59		96.9	97.8	97.3
62	Other	AUR	AurA	Aurora Kinase B	AurA	37		94.6	93.5	94
63	Other	CAMKK	Meta	Metazoanspecific family of CAMK (Calcium/Calmodulin Regulated Kinase) Kinase	CaMKK1 CaMKK2	17		100	94.7	97.4
64	Other	IKK		I kappa Kinase	IKKa IKKb IKKe TBK1	86		91	95.8	93.4
65	Other	NEK		Mitotic Kinase family, also known as NRK (NimARelated Kinase, based on Aspergillus NimA)	NEK1 NEK2 NEK3 NEK4 NEK5 NEK6 NEK7 NEK8 NEK9 NEK10 NEK11	33		82.2	86.7	84.5
66	Other	PEK	PKR	eukaryotic translation initiation factor 2-alpha kinase	PKR	13		83.8	94.6	89.2
67	Other	PLK		Polo Like Kinases	PLK1 PLK2 PLK3 PLK4	118		83.1	85.6	84.4
68	Other	TTK		TTK protein kinase	TTK	13		83.8	88.5	86.1
69	Other	ULK		unc-51-like kinase 1 (C. elegans)	ULK1 ULK3	10		90	100	95
70	Other	WEE		Homologs of yeast Wee1; cell cycle kinase	Wee1 Wee1B MYT1	14		100	100	100
71	Other	Wnk		With No Lysine kinases. Missing the canonical catalytic lysine, instead using a nearby lysine for catalysis	Wnk1 Wnk2 Wnk3 Wnk4	14		93.6	93.6	93.6
72	STE	STE11	MAP3K	MAP3K (MAP kinase kinase kinase) genes, homologous to yeast Ste 11	MAP3K1 MAP3K2 MAP3K3 MAP3K4 MAP3K5 MAP3K6 MAP3K7 MAP3K8	31		90.3	90.3	90.3
73	STE	STE20	PAK	p21Activated protein Kinase	PAK1 PAK2 PAK3 PAK4 PAK5 PAK6	116		89.7	93.9	91.8
74	STE	STE20	PAKB	p21(CDKN1A)-activated kinase	PAK4 PAK5 PAK6	14		92.1	90	91.1
75	STE	STE20	PAKA	p21 (CDKN1A)-activated kinase	PAK1 PAK2 PAK3	90		91.1	92.9	92
76	STE	STE20	YSK	serine/threonine kinase 24 (STE20 homolog, yeast)	MST3 MST4 YSK1	6		100	100	100
77	STE	STE20	KHS	mitogen-activated protein kinase kinase kinase kinase	GCK HPK1	7		74.3	84.3	79.3
78	STE	STE20	MSN	Nik related kinase	HGK MINK NRK TNIK	9		100	100	100
79	STE	STE20	MST	serine/threonine kinase 3 (STE20 homolog, yeast)	MST1 MST2	55		89.8	93.4	91.6
80	STE	STE7	MAP2K	MAP2K (MAP kinase kinase) genes, homologous to yeast Ste 7	MAP2K1 MAP2K2 MAP2K3 MAP2K4 MAP2K5 MAP2K6 MAP2K7	101		78.9	82.5	80.7
81	TK	Abl		Abelson murine leukemia homolog	ABL1(Abl) ABL2(ARG)	121		85.1	92.6	88.8
82	TK	Ack		tyrosine kinase, non-receptor	ACK	10		91	91	91
83	TK	Axl		AXL receptor tyrosine kinase	AXL MER TYRO3	10		88	88	88
84	TK	Csk		CSRC kinase	CSK CTK	29		89.6	93.1	91.4
85	TK	DDR		discoidin domain receptor family, member	DDR1 DDR2	20		76.2	84	80.1
86	TK	EGFR		Epidermal Growth Factor Receptor	EGFR HER2/ErbB2 HER3/ErbB3 HER4/ErbB4	79		90.1	91.4	90.8
87	TK	Eph		Ephrin receptors	EphA1 EphA2 EphA3 EphA4 EphA5 EphA6 EphA7 EphA8 EphA10 EphB1 EphB2 EphB3 EphB4 EphB5 EphB6	32		96.6	94.5	95.6
88	TK	FAK		Focal Adhesion Kinase	FAK PYK2	28		92.8	92.8	92.8
89	TK	Fer	Fer	Protooncogene tyrosineprotein kinase FER	FER	14		92.8	92.8	92.8
90	TK	Fer	Fes	Protooncogene tyrosineprotein kinase Fes/Fps	FES	11		90.9	90.9	90.9
91	TK	FGFR		Fibroblast Growth Factor Receptor	FGFR1 FGFR2 FGFR3 FGFR4	43		88.4	88.4	88.4
92	TK	InsR		Insulin Receptor and associated Kinases	INSR IRR	51		91.6	88.6	90.1
93	TK	InsR	IGF1R	Insulinlike growth factor I receptor	IGF1R	26		88.5	92.3	90.4
94	TK	JAK	JakA	First (active) kinase domain of the dualdomain Janus Kinases	JAK1 JAK2 JAK3	59		88.3	90.1	89.2
95	TK	TYK	TYK2	Nonreceptor tyrosineprotein kinase	TYK2	20		90	94	92
96	TK	Met	Met	Met protooncogene tyrosine kinase	MET RON	31		93.2	95.2	94.2
97	TK	PDGFR		Plateletderived growth factor	PDGFRa PDGFRb	43		92.1	90.2	91.1
98	TK	PDGFR	Fms	Plateletderived growth factor	FMS	15		96	96	96
99	TK	PDGFR	Kit	Plateletderived growth factor	KIT	8		100	100	100
100	TK	Ret		Protooncogene tyrosineprotein kinase receptor ret	RET	18		86.5	92.7	89.6
101	TK	Src	Brk	Protooncogene tyrosineprotein kinase BRK	BRK	22		94.9	95	95
102	TK	Src	Fgr	Protooncogene tyrosineprotein kinase FGR	FGR	9		100	94.4	97.2
103	TK	Src	Fyn	Protooncogene tyrosineprotein kinase FYN	FYN	66		85.9	90.2	88
104	TK	Src	HCK	Protooncogene tyrosineprotein kinase HCK	HCK	18		88.3	93.9	91.1
105	TK	Src	Lck	Protooncogene tyrosineprotein kinase Lck	LCK	64		92	93.2	92.6
106	TK	Src	LYN	Tyrosineprotein kinase LYN	LYN	73		91.5	91.5	91.5
107	TK	Src	Src	Protooncogene tyrosineprotein kinase Src	SRC	221		89.8	90.2	90
108	TK	Src	YES	Protooncogene tyrosineprotein kinase YES	YES	6		100	100	100
109	TK	Syk	SYK	Spleen tyrosine kinase	SYK	61		94.5	93.3	93.9
110	TK	Syk	ZAP70	70 kDa zetaassociated protein, Sykrelated tyrosine kinase	ZAP70	21		91.4	93.3	92.4
111	TK	Tec		BMX non-receptor tyrosine kinase	BMX ITK TEC TXK BTK	47		92.8	95.9	94.3
112	TK	Tec	BTK	Bruton tyrosine kinase	BTK	19		89.5	89.5	89.5
113	TK	Tec	ITK	IL2-inducible T-cell kinase	ITK	9		88.9	88.9	88.9
114	TK	Tec	TXK	Tyrosineprotein kinase TXK	TXK TEC	8		100	100	100
115	TK	Tie		Tunica interna endothelial cell kinase	TIE1 TIE2	9		100	100	100
116	TK	Trk		Neurotrophin receptor tyrosine kinases	TRKA TRKB TRKC	17		89.4	96.4	92.9
117	TK	VEGFR		Vascular Endothelial Growth Factor Receptors	FLT1 FLT4 FLT3 KDR	36		88.5	92.3	90.4
118	TKL	IRAK		interleukin-1 receptor-associated kinase	IRAK1 IRAK4	9		100	92.9	96.5
119	TKL	RAF		RAF protooncogene serine/threonineprotein kinase	RAF1 ARAF BRAF KSR1 KSR2	9		66.7	77.8	72.3
120	TKL	STKR	Type1	Serine/Threonine Kinase Receptors; receptors for activin and TGFb ligands. Type 1	ALK4 BMPR1B TGFbR1	17		91.2	91.2	91.2
121	TKL	STKR	Type2	Serine/Threonine Kinase Receptors; receptors for activin and TGFb ligands. Type 2	TGFbR2	11		98.9	99.6	99.3
122	TKL	STKR	TGFbR	Serine/Threonine Kinase Receptors; receptors for activin and TGFb ligands.	TGFbR1 TGFbR2	20		94	95	94.5